Genomic DNA is fragmented into random pieces and cloned as a bacterial library. DNA from individual bacterial clones is sequenced and the sequence is assembled by using overlapping DNA regions.
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Typically, gene synthesis technologies produce short strands of DNA called oligonucleotides that must be assembled into larger, more useful fragments. Conntemporary gene assembly methods, either performed by expensive automatic industrial robots in contract-synthesis companies (gene foundries) or manually by error-prone humans in laboratories, involve time-consuming, multi-step processes that are ultimately inefficient uses of time, resources and manpower. Without an easier means of assembling DNA, the clinical benefits of contemporary DNA sequencing breakthroughs are unlikely to be fully realized. There are a variety of DNA assembly technologies and techniques to overcome limitations.
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