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Genomic DNA is fragmented into random pieces and cloned as a bacterial library. DNA from individual bacterial clones is sequenced and the sequence is assembled by using overlapping DNA regions. © 2007 Wikipedia
Typically, gene synthesis technologies produce short strands of DNA called oligonucleotides that must be assembled into larger, more useful fragments. Conntemporary gene assembly methods, either performed by expensive automatic industrial robots in contract-synthesis companies (gene foundries) or manually by error-prone humans in laboratories, involve time-consuming, multi-step processes that are ultimately inefficient uses of time, resources and manpower. Without an easier means of assembling DNA, the clinical benefits of contemporary DNA sequencing breakthroughs are unlikely to be fully realized. There are a variety of DNA assembly technologies and techniques to overcome limitations.
 

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About the authors

View full profile Edward Perello from London

Edward Perello is the founder of Desktop Genetics, a company at the forefront of CRISPR genome editing technology. His team is working to provide researchers with access to state of the art genome engineering capabilities from their computers and create an AI that can predict optimal genome editing solutions in any organism.

Edward is a SynBio LEAP fellow working to get more non-biologists into the field.

https://www.deskgen.com/

View full profile Jérôme Lutz from Berlin & Munich, Germany

I like to share the great things I discover daily while researching and working in the field of Synthetic Biology.

When I talk to people about it, they often refer to Science Fiction. However, when I send them links to this wiki and they read through those pages, they start understanding that this is real and it's happening right now.

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