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© Image 2013 iGem TU Munich
The iGem Team says Hallo and Explains their product © 2013 iGem

The PyscoFilter removes antibiotics, hormones and various noxious substances from water. It was an iGEM project from the TU Munich in 2013. They have developed a device to implement the filter in an aquatic environment, investigated the application of this new technology and examined its economic feasibility. The PhyscoFilter may become a game-changing approach to improve global water quality in an affordable and sustainable fashion.


How to implement such a filter?

 
Small filter example built by the team © 2013 iGem 2012 TU Munich

Our open pond model consists of meander shaped perspex and two threads to adjust the pond's pitch. Therefore different flow speeds can be implemented. The floor of our open pond is lined with agar to grow the moss on. The pond's lid can be taken off to remove the moss.

The open pond implementation can be used as the last step of a wastewater treatment plant. Even though the contact surface between moss and water might not be as big as in the tube reactor, an implementation with membrane bound effectors is still thinkable. Using membrane bound effectors has the advantage that an emission of effectors into the clean water can be avoided almost entirely. Yet a secretion of effectors to the water may accomplish the biodegradation more effectively.

Read more on possible implementations at http://2013.igem.org/Team:TU-Munich/Results/Implementation

© Text 2013 iGem Wiki TU Munich
 

Construction plan

Overview over the BioBricks used in TU-Munich 2013 iGEM project © 2013 iGem 2013 TU Munich

BioBricks used

These are the biobricks that the team has put together in order to change the functionality of the original moss. If you click the links, you will be able to download the DNA sequence of each of those bricks and start assembling them yourself. The length column indicates how many DNA base pairs each brick has.

NameTypeDescriptionDesignerLength
BBa_K1159000CodingErythromycin Esterase Type II (EreB) in RFC[25]Louise FUNKE1254
BBa_K1159001CodingNanoLuc Luciferase in RFC[25]TU Munich 2013510
BBa_K1159002CodingLaccase from Bacillus pumilus in RFC[25]Andreas-David BRUNNER1524
BBa_K1159004CodingHuman protein phosphatase 1 (PP1) in RFC[25]TU Munich 2013972
BBa_K1159006CodingSecretory NanoLuc Luciferase (IgKappa-SigP_nLuc) in RFC[25] N-PartTU Munich 2013583
BBa_K1159010CodingSecretory NanoLuc Luciferase (SERK-SigP_nLuc) in RFC[25] N-PartTU Munich 2013616
BBa_K1159112CodingFRET Reporter for TEV Protease activity (eCFP_TEV-site-linker_SYFP2) in RFC[25]Dong-Jiunn Jeffery TRUONG1467
BBa_K1159200CodingSplitted and engineered N-terminal FbaB for isopeptide bound formation (SpyCatcher) in RFC[25]Dong-Jiunn Jeffery TRUONG339
BBa_K1159201CodingSplitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]Dong-Jiunn Jeffery TRUONG39
BBa_K1159003CodingEngineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]TU Munich 2013522
BBa_K1159005CodingSecretory Erythromycin Esterase Type II (IgKappa-SigP_EreB) in RFC[25] N-PartLouise FUNKE1327
BBa_K1159007CodingSecretory Laccase (IgKappa-SigP_Laccase) in RFC[25] N-PartAndreas-David BRUNNER1597
BBa_K1159008CodingSecretory Catechol-2,3-dioxygenase (IgKappa-SigP_XylE) in RFC[25] N-PartRosario CICCONE991
BBa_K1159009CodingSecretory Erythromycin Esterase Type II (SERK-SigP_EreB) in RFC[25] N-PartLouise FUNKE1360
BBa_K1159011CodingSecretory Laccase (SERK-SigP_Laccase) in RFC[25] N-PartAndreas-David BRUNNER1630
BBa_K1159012CodingSecretory Catechol-2,3-dioxygenase (SERK-SigP_XylE) in RFC[25] N-PartRosario CICCONE1024
BBa_K1159013CodingSecretory Human Protein Phosphatase 1 (SERK-SigP_PP1) in RFC[25] N-PartTU Munich 20131078
BBa_K1159014CodingMembrane-anchored Erythromycin Esterase Type II in RFC[25] N-PartLouise FUNKE2362
BBa_K1159016CodingMembrane-anchored Laccase in RFC[25] N-PartAndreas-David BRUNNER2632
BBa_K1159017CodingMembrane-anchored FluA triple mutant in RFC[25] N-PartTU Munich 20131630
BBa_K1159018CodingMembrane-anchored Catechol-2,3-dioxygenase (XylE) in RFC[25] N-PartRosario CICCONE2026
BBa_K1159019CodingMembrane-anchored Human Protein Phosphatase 1 (PP1) in RFC[25] N-PartTU Munich 20132080
BBa_K1159100CodingAutolysis resistant TEV Protease S219V mutant in RFC[25]Dong-Jiunn Jeffery TRUONG708
BBa_K1159101CodingN-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]Dong-Jiunn Jeffery TRUONG354
BBa_K1159102CodingC-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]Dong-Jiunn Jeffery TRUONG354
BBa_K1159103CodingN-terminal Phytochrome Interacting Factor 3 (PIF3) in RFC[25]Dong-Jiunn Jeffery TRUONG297
BBa_K1159104CodingN-terminal Phytochrome Interacting Factor 6 (PIF6) in RFC[25]Dong-Jiunn Jeffery TRUONG297
BBa_K1159105CodingMature Nuclease NucA from Staphylococcus aureus (Thermonuclease) in RFC[25]Dong-Jiunn Jeffery TRUONG447
BBa_K1159106CodingPhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a light-switchable TEV-Protease) in RFC[25]Dong-Jiunn Jeffery TRUONG3195
BBa_K1159107CodingN-TEV-Protease_36AALinker_PIF3 (Second part for a light-switchable TEV-Protease) in RFC[25]Dong-Jiunn Jeffery TRUONG771
BBa_K1159108CodingN-TEV-Protease_36AALinker_PIF6 (Second part for a light-switchable TEV-Protease) in RFC[25]Dong-Jiunn Jeffery TRUONG771
BBa_K1159109CodingMicrococcal nuclease (Thermonuclease) with N-terminal SV40 NLS in RFC[25]Dong-Jiunn Jeffery TRUONG510
BBa_K1159110CodingThermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]Dong-Jiunn Jeffery TRUONG618
BBa_K1159111CodingMembrane-associated Thermonuclease NucA with TEV site and SV40 NLS in RFC[25] N-PartDong-Jiunn Jeffery TRUONG916
BBa_K1159113Translational_UnitEukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based)Dong-Jiunn Jeffery TRUONG4625
BBa_K1159114Translational_UnitEukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based)Dong-Jiunn Jeffery TRUONG4625
BBa_K1159115Translational_UnitEukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based) with downstream IRESDong-Jiunn Jeffery TRUONG5269
BBa_K1159116Translational_UnitEukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based) with downstream IRESDong-Jiunn Jeffery TRUONG5269
BBa_K1159117Translational_UnitRed light triggered Kill Switch for plants translation unit (PhyB/PIF3 version)Dong-Jiunn Jeffery TRUONG6191
BBa_K1159118Translational_UnitRed light triggered Kill Switch for plants translation unit (PhyB/PIF6 version)Dong-Jiunn Jeffery TRUONG6191
BBa_K1159119Translational_UnitRed light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)Dong-Jiunn Jeffery TRUONG6751
BBa_K1159120Translational_UnitRed light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)Dong-Jiunn Jeffery TRUONG6751
BBa_K1159202CodingSecretory SpyCatcher (IgKappa-SigP_SpyCatcher) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG412
BBa_K1159203CodingSecretory SpyTag (IgKappa-SigP_SpyTag) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG112
BBa_K1159204CodingSecretory SpyCatcher (SERK-SigP_SpyCatcher) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG445
BBa_K1159205CodingSecretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG145
BBa_K1159206CodingMembrane-anchored SpyCatcher in RFC[25] N-PartDong-Jiunn Jeffery TRUONG1447
BBa_K1159207CodingMembrane-anchored SpyTag in RFC[25] N-PartDong-Jiunn Jeffery TRUONG1147
BBa_K1159208CodingSecretory SpyTag_nLuc fusion (IgKappa-SigP_SpyTag_nLuc) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG628
BBa_K1159209CodingSecretory nLuc_SpyTag fusion (IgKappa-SigP_nLuc_SpyTag) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG628
BBa_K1159210CodingSecretory nLuc_SpyCatcher fusion (IgKappa-SigP_nLuc_SpyCatcher) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG928
BBa_K1159211CodingSecretory SpyCatcher_nLuc fusion (IgKappa-SigP_SpyCatcher_nLuc) in RFC[25] N-PartDong-Jiunn Jeffery TRUONG928
BBa_K1159212Translational_UnitMembrane-anchored SpyCatcher with secretory SpyTag_nLuc reporter fusion translational unitDong-Jiunn Jeffery TRUONG2725
BBa_K1159213Translational_UnitMembrane-anchored SpyCatcher with secretory nLuc_SpyTag reporter fusion translational unitDong-Jiunn Jeffery TRUONG2725
BBa_K1159214Translational_UnitMembrane-anchored SpyTag with secretory nLuc_SpyCatcher reporter fusion translational unitDong-Jiunn Jeffery TRUONG2725
BBa_K1159215Translational_UnitMembrane-anchored SpyTag with secretory SpyCatcher_nLuc reporter fusion translational unitDong-Jiunn Jeffery TRUONG2725
BBa_K1159300RBSPolioviral Internal Ribosome Entry Site (IRES) for polycistronic expression in eukaryotesDong-Jiunn Jeffery TRUONG627
BBa_K1159301CodingSuper Yellow Fluorescent Protein 2 (SYFP2) in RFC[25]Dong-Jiunn Jeffery TRUONG714
BBa_K1159302CodingEnhanced Cyan Fluorescent Protein (eCFP) in RFC[25]Dong-Jiunn Jeffery TRUONG714
BBa_K1159303Protein_DomainSignal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]TU Munich 2013100
BBa_K1159304Protein_DomainSignal Peptide of Ig Kappa chain from Mus musculus in RFC[25] N-partTU Munich 201367
BBa_K1159305Protein_DomainTransmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]TU Munich 2013186
BBa_K1159307Terminator35S Terminator of Cauliflower Mosaic Virus (CaMV)TU Munich 2013217
BBa_K1159308DeviceNeomycin phosphotransferase II (nptII) plant expression cassette for G418 resistanceDong-Jiunn Jeffery TRUONG1517
BBa_K1159309Protein_Domain19 AA Linker with N-terminal Strep-tag II and C-terminal TEV cleavage site in RFC[25]TU Munich 201357
BBa_K1159310Protein_Domain34 AA flexible linker with C-terminal TEV cleavage site (GGGGS)x5-TEV-site-linker) in RFC[25]Dong-Jiunn Jeffery TRUONG102
BBa_K1159314CodingTMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-PartDong-Jiunn Jeffery TRUONG292
BBa_K1159315CodingTMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]TU Munich 2013933
BBa_K1159316CodingSERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]TU Munich 2013996
BBa_K1159317DevicepRD29A_Strong-Plant-Kozak-(RBS)_RFP_t35S_nptII-cassetteTU Munich 20133285
BBa_K1159015CodingMembrane-anchored NanoLuc Luciferase in RFC[25] N-PartTU Munich 20131585
BBa_K1159306RegulatoryActin5 Promoter from Physcomitrella patensIngmar Polte1948
BBa_K1159311CodingGFPmut1TU Munich 2013711
BBa_K1159312TagN-terminal Strep-tag-II in RFC[25] N-PartTU Munich 201339
BBa_K1159313TagN-terminal His6-tag in RFC[25] N-PartDong-Jiunn Jeffery TRUONG42
BBa_K1159318CodingTEV cleavage site flanked by Gly for better accessibility in RFC[25]Dong-Jiunn Jeffery TRUONG27
BBa_K1159991DNA10/10 NC Assembly ScarTU Munich 20138
BBa_K1159992DNA10/10 C Assembly ScarTU Munich 20136
BBa_K1159993DNA25/25 Assembly ScarTU Munich 20136
BBa_K1159994DNA10/25 Assembly ScarTU Munich 201315
BBa_K1159995DNA10/25 N-Part Assembly ScarTU Munich 20136
BBa_K1159996DNA25/10 NC Assembly ScarTU Munich 201317

Latest news

The latest news were that they won a couple of awards, however since mid 2014 there were no new announcements made on Facebook

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About the author

View full profile Jérôme Lutz from Berlin & Munich, Germany

I like to share the great things I discover daily while researching and working in the field of Synthetic Biology.

When I talk to people about it, they often refer to Science Fiction. However, when I send them links to this wiki and they read through those pages, they start understanding that this is real and it's happening right now.
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